freezone 2 5 l 50 ◦c lyophilizer  (Labconco)

Journal: Biotechnology Reports

Article Title: Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert

doi: 10.1016/j.btre.2026.e00950

Figure Lengend Snippet: Microencapsulated Lacticaseibacillus rhamnosus (MLR) with sodium alginate in first layer and Qodume shirazi seed mucilage (QCSM), Camelina satvia seed mucilage (CSSM) as second layer in double layers by extrusion method. Photographic images of MLR at varying concentrations of QCSM and CSSM: (a) 0 % QCSM and 100 % CSSM; (b) 30 % QCSM and 70 % CSSM; (c) 50 % QCSM and 50 % CSSM; (d) 70 % QCSM and 30 % CSSM; (e) 100 % QCSM and 0 % CSSM. Microscopy images (40X) of MLR at the corresponding concentration ratios: (f) 0 % QCSM and 100 % CSSM; (g) 30 % QCSM and 70 % CSSM; (h) 50 % QCSM and 50 % CSSM; (i) 70 % QCSM and 30 % CSSM; (j) 100 % QCSM and 0 % CSSM. Scanning electron microscopy (SEM) images of MLR at the corresponding concentration ratios: (k) 0 % QCSM and 100 % CSSM; (l) 30 % QCSM and 70 % CSSM; (m) 50 % QCSM and 50 % CSSM; (n) 70 % QCSM and 30 % CSSM; (o) 100 % QCSM and 0 % CSSM respectively used in encapsulation.

Article Snippet: Lyophilized L. rhamnosus ATCC 53,103 was obtained from the Persian Type Culture Collection, Tehran, Iran.

Techniques: Microscopy, Concentration Assay, Electron Microscopy, Encapsulation

Journal: Biotechnology Reports

Article Title: Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert

doi: 10.1016/j.btre.2026.e00950

Figure Lengend Snippet: Survival of free Lacticaseibacillus rhamnosus (FLR) and microencapsulated Lacticaseibacillus rhamnosus (MLR) under various conditions. Microencapsulation was performed using a double-layer extrusion method with sodium alginate in the first layer and varying ratios of Qodume shirazi seed mucilage (QCSM), Camelina satvia seed mucilage (CSSM) in the second layer. MLR formulations were prepared with QCSM: CSSM ratios of: MLR-QC1 (0:100), MLR-QC2 (30:70), MLR- QC3 (50:50), MLR- QC4 (70:30), and MLR- QC5 (100:0) percent of QCSM and CSSM, respectively used in encapsulation. Viable count numbers of LR are shown at (a) 72 °C, (b) during storage time. 1. Data (mean ± standard error) are from three replications ( n = 3).

Article Snippet: Lyophilized L. rhamnosus ATCC 53,103 was obtained from the Persian Type Culture Collection, Tehran, Iran.

Techniques: Encapsulation

Journal: Biotechnology Reports

Article Title: Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert

doi: 10.1016/j.btre.2026.e00950

Figure Lengend Snippet: Changes in (a) pH and (b) acidity values of free Lacticaseibacillus rhamnosus (FLR), microencapsulated LR with formulation QC3 (MLR-QC3) containing a 50:50 ratio of Qodume shirazi seed mucilage (QCSM): Camelina satvia seed mucilage (CSSM), and control dessert (C) during storage time. Microencapsulation was performed using a double-layer extrusion method, with sodium alginate in the first layer and QCSM: CSSM in the second layer. 1.Data (mean ± standard error) are from three replications( n = 3).

Article Snippet: Lyophilized L. rhamnosus ATCC 53,103 was obtained from the Persian Type Culture Collection, Tehran, Iran.

Techniques: Formulation, Control

Journal: Biotechnology Reports

Article Title: Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert

doi: 10.1016/j.btre.2026.e00950

Figure Lengend Snippet: The viable count of Lacticaseibacillus rhamnosus (FLR), microencapsulated LR with formulation QC3 (MLR-QC3) containing a 50:50 ratio of Qodume shirazi seed mucilage (QCSM): Camelina satvia seed mucilage (CSSM), and control dessert (C) during storage time. Microencapsulation was performed using a double-layer extrusion method, with sodium alginate in the first layer and QCSM: CSSM in the second layer. (a) Viable count during storage time and (b) Viable count under simulated gastrointestinal conditions in 1 St and 14th of storage. 1.Data (mean ± standard error) are from three replications( n = 3).

Article Snippet: Lyophilized L. rhamnosus ATCC 53,103 was obtained from the Persian Type Culture Collection, Tehran, Iran.

Techniques: Formulation, Control

Journal: Biotechnology Reports

Article Title: Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert

doi: 10.1016/j.btre.2026.e00950

Figure Lengend Snippet: Scanning electron microscopy (SEM) images of goat milk dessert (GMD) containing Lacticaseibacillus rhamnosus (FLR), microencapsulated LR with formulation QC3 (MLR-QC3) containing a 50:50 ratio of Qodume shirazi seed mucilage (QCSM): Camelina satvia seed mucilage (CSSM), and control dessert (C) during storage time. Microencapsulation was performed using a double-layer extrusion method, with sodium alginate in the first layer and QCSM: CSSM in the second layer. (a) C on day 1; (b) FLR on day 1; (c) MLR- QC3 on day 1; (d) C on day 21; (e) FLR on day 21; (f) MLR- QC3 on day 21 of storage time.

Article Snippet: Lyophilized L. rhamnosus ATCC 53,103 was obtained from the Persian Type Culture Collection, Tehran, Iran.

Techniques: Electron Microscopy, Formulation, Control